Molecular Biology Plataform

The Molecular Biology Platform offers the Research Community access to an excellent custom molecular biology service. This Platform has designed a streamlined process that is able to generate a huge variety of DNA constructs, viral and non-viral, fast, efficiently, and inexpensively. It also provides recombinant protein production.

Molecular Biology Service price

Subcloning cDNA/DNA PCR cloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Cloning (≤ 500 bp) 150-300€
DNA Cloning (501 bp -1.5 kb) 200-500€
DNA Cloning (≥1.5 kb) 40€/h

Subcloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Subcloning (≤2,000 bp) 200-300€
DNA Subcloning (>2,000 bp) 40€/h

Site-directed mutagenesis
Site-directed mutagenesis (1-2 reactions/construct) 300-400€
Site-directed mutagenesis (3-5 reactions/construct) 400-500€

cDNA/DNA PCR cloning (Gateway®)
DNA PCR cloning into pENTR (≤2,000 bp) (eg pCR8) 200-300€
DNA PCR cloning into pENTR (>2,000 bp) 40€/h
Subcloning into prokaryotic or mammalian vectors, several tags available
Subcloning into lentiviral vectors (vectors available pLenti6, pLenti6/CMV) 200-300€
Subcloning into adenoviral vectors (vectors available pAd, pAd/CMV) 300-500€

Virus production
Lentivirus production 300-400€
Adenovirus production 200-500€

Protein production (prokaryotic system) 500-1000€
Protein production (eukaryotic system) 40€/h

Other services available

(all reagents/materials and consumables are included)

Contact the Coordinator by email/telephone

Scientific Coordinator

José Ramalho
Researcher

Facility Manager

José Ramalho
Researcher

Team

Carin Didoss
Sergio Muñoz Cobos
Hakan Onur